Crystal structure of human 8-oxoguanine glycosylase K249H mutant bound to the reaction intermediate derived from the crystal soaked into the solution at pH 4.0 under 298 K for 3 weeks

Crystal structure of human 8-oxoguanine glycosylase K249H mutant bound to the reaction intermediate derived from the crystal soaked into the solution at pH 4.0 under 298 K for 3 weeks Descriptor: DNA (5'-D(*AP*GP*CP*GP*TP*CP*CP*A)-3'), DNA (5'-D(*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3'), DNA (5'-D(P*GP*TP*CP*TP*AP*CP*C)-3'), ... Authors: Unno, M, Koga, M, Minowa, N, Komuro, S, Tanaka, Y. Deposit date: 2024-01-18 Release date: 2025-07-23 Last modified: 2025-08-13 Method: X-RAY DIFFRACTION (1.7 Å) Cite: Capturing a glycosylase reaction intermediate in DNA repair by freeze-trapping of a pH-responsive hOGG1 mutant. Nucleic Acids Res., 53, 2025

人类8-氧鸟嘌呤糖苷酶（8-oxoguanine glycosylase）K249H突变体与反应中间体结合的晶体结构，该中间体源自于在pH 4.0、298 K条件下于溶液中浸泡3周的晶体。
描述：DNA (5'-D(*AP*GP*CP*GP*TP*CP*CP*A)-3')、DNA (5'-D(*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3')、DNA (5'-D(P*GP*TP*CP*TP*AP*CP*C)-3')……
作者：Unno M、Koga M、Minowa N、Komuro S、Tanaka Y
沉积日期：2024-01-18
发布日期：2025-07-23
末次修改日期：2025-08-13
实验方法：X射线衍射（分辨率1.7 Å）
引用：通过冷冻捕获pH响应型hOGG1突变体以捕捉DNA修复过程中的糖苷酶反应中间体。《核酸研究》（Nucleic Acids Res.）, 53, 2025