RNA sequencing of spheroids from HUVECs for differences in Cis- and Trans-signaling of IL-11

Cytokines of the IL-6 family, such as IL-11, may influence angiogenesis. Our experiments showed opposite angiogenic effects on HUVECs depending on cis- or transsignaling of IL-11. Spheroids from the 3D angiogenesis model (spheroid sprouting assay) were lysed in QIAzole buffer by vigorous vortexing. The transcriptomic changes were analyzed by RNA sequencing. Overall design: Spheroids from the spheroid sprouting assays were lysed in QIAzole buffer. A total of 12 samples from three independent spheroid assays were analyzed under the following conditions: VEGF, IL-11+VEGF, sIL-11R+VEGF, IL-11+sR+VEGF