Structure-guided mutagenesis reveals an essential E92-mediated interaction network in the F1 capsule regulator Caf1R of Yersinia pestis

Title: Structure-guided mutagenesis reveals an essential E92-mediated interaction network in the F1 capsule regulator Caf1R of Yersinia pestis. This Zenodo record contains the raw data and supporting materials associated with the manuscript "Structure-guided mutagenesis reveals an essential E92-mediated interaction network in the F1 capsule regulator Caf1R of Yersinia pestis". The deposited files include raw SDS-PAGE and Western blot images, GraphPad Prism project files, source numerical data, and supplementary figures and datasets used in the study. These materials support the analyses and conclusions presented in the manuscript and are provided to promote transparency, reproducibility, and independent verification of the reported findings. The dataset specifically supports the structural and functional characterisation of the Caf1R regulator and the identification of an essential E92-mediated interaction network involved in F1 capsule regulation in Yersinia pestis. Copyright Statement © 2026 Kumar D. Gahlot. All files deposited in this Zenodo record, including raw experimental data, source data, images, GraphPad Prism files, and supplementary materials, remain the intellectual property of the author. The materials are distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits sharing, adaptation, distribution, and reproduction in any medium or format, provided appropriate credit is given to the original author and source. Keywords Yersinia pestis Caf1R F1 capsule AraC/XylS regulator Structure-guided mutagenesis Protein regulation Bacterial pathogenesis Virulence factors Research data repository SDS-PAGE Western blot Raw data GraphPad Prism