Data for: Computational flow cytometry of planktonic populations for the evaluation of biofouling control programs in district cooling plants

The data attached here represent the core data for a 2019 flow cytometry (FCM)-centered study to evaluate biofouling control programs at 3 full-scale district cooling plants (DCPs) in the Middle East. The data shows raw flow cytometry results from staining water samples from these plants with SYBR Green 1 (FL1 channels) or propidium iodide (FL3 channels). Staining with SYBR Green 1 was used to discern between planktonic cells and debris in the sample, whereas propidium iodide was used to enumerate membrane-compromised cells. This data can be used to calculate the number of intact (essentially living) cells in the samples which , when combined with the below metadata, can be used to reveal spatiotemporal dynamics in planktonic counts of biofouling communities. From these dynamics, an evaluation of the biofouling management practices at the study DCPs can be made. The data is compatible with computational FCM pipelines in R and the results of a computational FCM analysis have been submitted for publication under the above title.

DCP_FCM_data.zip - The data consist of 1,244 flow cytometry files in FCS format. FCS files were generated on an Accuri C6 Plus flow cytometer. FCS files represent samples collected from three locations (designated A- make-up water faucets, B - water cooling towers and C - condenser outlet waters) from each of 3 district cooling plants (designated KC, RB and YI) daily over a 5 week sampling period in 2019. Files are named based on the position of the sample in the Accuri workspace file (indicated by an alphanumeric, e.g. B01) followed by our lab's standardized sample ID conventions which take the format [Plant]-[site]-[sample type]-[status]-[sample no.] and are annotated based on the stain applied as follows: "-SG.fcs" are samples stained with 1 x SYBR Green 1, "-PI.fcs" are stained with 6 micromolar propidium iodide and "-US.fcs" are unstained control samples. File names also indicate whether the sampling point was A- active (in use) or D- deactive (on standby) at the time of sampling and "W"- simply indicates that the samples are of type "water".

DCP_metadata.csv - Metadata describing the DCPs from where the samples were taken to contextualize the flow cytometry data for analysis in R. This data is composed of plant metadata taken directly from the SCADA systems on site and water chemistry data that was acquired in lab (TIC, TOC, conductivity and pH). Sample name column matches those of the FCS files and can be used to assign this metadata in R. "NA"- indicates where the data point was not available.

此处附上的数据集构成了2019年一项以流式细胞术（FCM）为核心的研究的核心数据，旨在评估中东地区3个全规模区域冷却站（DCP）的生物污垢控制方案。数据展示了从这些设施中提取的水样经SYBR Green 1（FL1通道）或碘化丙啶（FL3通道）染色后的原始流式细胞术结果。使用SYBR Green 1染色用于区分样品中的浮游细胞和碎片，而碘化丙啶则用于计数膜受损细胞。这些数据可用于计算样品中完整（实质上为活细胞）的细胞数量，结合下述元数据，可揭示生物污垢群落浮游计数在时空动态上的变化。通过分析这些动态，可以对研究DCP的生物污垢管理实践进行评估。数据与R中的计算流式细胞术管道兼容，且基于计算流式细胞术分析的结果已提交以该标题发表。

DCP_FCM_data.zip - 数据包括1,244个FCS格式的流式细胞术文件。这些FCS文件是在Accuri C6 Plus流式细胞仪上生成的。FCS文件代表从3个区域冷却站（分别标记为KC、RB和YI）的3个位置（标记为A-补充水龙头、B-水冷却塔和C-冷凝器出口水）在2019年5周采样期内每日收集的样本。文件命名基于样本在Accuri工作空间文件中的位置（由字母数字标识，例如B01），随后是本实验室的标准样本ID规范，其格式为[Plant]-[site]-[sample type]-[status]-[sample no.]，并根据所施加的染色进行标注："-SG.fcs"表示用1 x SYBR Green 1染色的样本，"-PI.fcs"表示用6微摩尔碘化丙啶染色的样本，"-US.fcs"表示未染色的对照样本。文件名还表明采样点在采样时是A-活跃（在使用中）或D-非活跃（备用），而"W"仅表示样本类型为“水”。

DCP_metadata.csv - 描述从采集样本的DCP的元数据，以便在R中分析流式细胞术数据。该数据由直接从现场SCADA系统获取的设施元数据和实验室获取的水化学数据（总离子浓度、总有机碳、电导率和pH值）组成。样本名称列与FCS文件中的样本名称相匹配，并可用于在R中分配此元数据。“NA”-表示数据点不可用。