Supporting data for "Immovably flexible: Spatial variation in emersion stress responses by <i>Saccostrea cuccullata</i>"

This item includes all measurements of the physiological and behavioural responses of <i>Saccostrea cuccullata </i>when they are exposed to emersion in summer; a hot and dry period where the oysters were exposed to direct solar radiation due to the receding of tides. Chapter 2 investigates the thermal environment and physical characteristics between the three study sites; at three vertical heights within the oyster band, and how these different thermal regimes may impact the survival and reproductive development of the oysters. Chapter 3 quantifies the thermal stress and desiccation that oysters experience during summer emersion periods by measuring the change in haemolymph oxygen tension, osmolality of bodily fluid (i.e. haemolymph and extra-pallial fluid) and body water content throughout the emersion period in the field and in a laboratory simulated emersion.Chapter 4 measures the thermal tolerance, cellular heat shock response (i.e. relative gene expression of hsp70) and associated energetic trade-offs in the oysters at different sites and shore heights. This chapter also investigate the potential plasticity in cellular heat shock response of oyster by comparing the cellular heat shock response between field fresh and common garden acclimated oyster.Chapter 5 explores potential behavioural responses exhibited by oysters by measuring their gaping behaviour during different seasons and tidal conditions.

本数据集涵盖了帽状牡蛎（Saccostrea cuccullata）在夏季暴露于退潮露出环境下的全部生理与行为响应测量数据；该时段为炎热干燥的夏季，牡蛎会因潮汐退却而暴露于直接日照之下。
第二章探究了三处研究样地（牡蛎带内三个垂直高度站位）的热环境与物理特征，并分析了不同热环境特征如何影响牡蛎的存活与生殖发育进程。
第三章通过野外与实验室模拟退潮暴露实验，全程测定了牡蛎在夏季退潮暴露期间的血淋巴（haemolymph）氧分压、体液（血淋巴与外套腔液（extra-pallial fluid））渗透压以及机体含水量变化，以此量化牡蛎所承受的热胁迫与干燥脱水压力。
第四章测定了不同样地与潮间带高度下牡蛎的热耐受能力、细胞热休克响应（即热休克蛋白70（hsp70）的相对基因表达水平）以及相关的能量权衡关系；本章还通过对比野外新鲜采集与同质园驯化牡蛎的细胞热休克响应，探究了牡蛎细胞热休克响应的潜在可塑性。
第五章通过测定不同季节与潮汐条件下牡蛎的贝壳张开行为，探究了其潜在的行为响应模式。

搜集汇总

背景与挑战

背景概述

该数据集支持一项关于Saccostrea cuccullata（牡蛎）在夏季暴露于空气时生理和行为响应空间变化的研究。数据包括多个章节的测量结果：热环境与物理特征、热应激与脱水量化、热耐受性与细胞热休克响应以及季节和潮汐条件下的行为响应，旨在揭示不同地点和岸高对牡蛎适应性的影响。