Ion torrent sequencing results of Atrogin-1 gene region among different chicken lines

To discover the SNPs information of Atrogin-1 gene region among chicken breeds, we targeted sequence 40 kb (chr 2: 143,690,694-143,730,638 bp) including upstream 10 kb and downstream 6 kb of this gene. RJF, Chahua, Tibetan, Silky, Fast Silky, mini, Cobb and AA were used (N = 50) (Chahua, Tibetan, Silky, Fast Silky and mini are Chinese native chicken breeds, while Cobb and AA are commercial broiler lines which derived from Cornish crosses). The PCR products were purified and subsequently sequenced by Ion torrent. Different chicken breeds were separated by barcode.