Spectrum of spontaneous rpoB-mutations obtained by method 2 (single colonies from 10-ml cultures).

Results for MTB72 (first row) and strains H103, 2001–2184 and 2001–2185 were obtained in the first experiment, where we assessed the influence of pre-existing INH resistance on the spectrum of mutations. Results for MTB72 (second row) and 9900098, 2001-1669 and 2001-1670 were obtained in the second experiment, where we determined the role of the LAM genotype on the spectrum of rpoB-mutations. Results for MTB72 (third row) and 9500592, 2002-1640, 2002-1585 and 17583 were obtained in the third experiment, where we determined the role of the Beijing genotype on the spectrum of rpoB-mutations. ins: insertion, indel: combined insertion/deletion, Δ: deletion.