Whole brain RNA-seq from maternal Astatotilapia burtoni

Brains from female Astatotilapia burtoni were sampled at four timepoints throughout the reproductive cycle with the primary goal of comparing transcriptome profiles at the different stages and identifying genes and networks involved in parental and fasting behaviors. Overall design: Females were maintained in 30 gallon mixed sex stock tanks with 2-4 males and 8-12 females. Tanks were checked daily, and when brooding females were captured and fry development stage was confirmed. Females were systematically assigned to one of four treatment groups: 2 days mouthbrooding (B02), 14 days mouthbrooding (B14), 2 days post-release of fry (R02), and 14 days post release (R14) (Fig 1). Only females confirmed to be holding stage 0 fry were assigned to the B02 group.