Friedhoff et al., Figure 4

Fig 4. SARS-CoV-2 replicon DNA-encoded luciferase expression is dependent upon DNA linearization but occurs in the absence of T7 RNAP and is insensitive to remdesivir. HEK293T cells transduced with LPCX(AB) or with LPCX(AB)-T7RNAP were PEI-transfected with pSMART-BAC-T7-scv2, linearized or not as indicated, and treated or not with remdesivir (RDV). Luciferase activity was quantified two days later as detailed in the Methods section, and is expressed in relative light units (RLU). Shown are averaged values from duplicate transfections with two luciferase assays done for each transfection. A Student’s t-test was used to assess statistical significance; *, P < 0.05; ns, non-significant.