Dataset supporting "Functional antibodies exhibit light chain coherence"

The vertebrate adaptive immune system modifies the genome of individual B cells to encode antibodies binding particular antigens. In most mammals, antibodies are composed of a heavy and a light chain which are sequentially generated by recombination of V, D (for heavy chains), J, and C gene segments. Each chain contains three complementarity-determining regions (CDR1-3), contributing to antigen specificity. Certain heavy and light chains are preferred for particular antigens. We considered pairs of B cells sharing the same heavy chain V gene and CDRH3 amino acid sequence and isolated from different donors, also known as public clonotypes. We show that for naive antibodies (not yet adapted to antigens), the probability that they use the same light chain V gene is ~10%, whereas for memory (functional) antibodies it is ~80%. This property of functional antibodies is a phenomenon we call light chain coherence.  We also observe it when similar heavy chains recur within a donor.  Thus, though naive antibodies appear to recur by chance, the recurrence of functional antibodies reveals surprising constraint and determinism in the processes of V(D)J recombination and immune selection. For most functional antibodies, the heavy chain determines the light chain.

脊椎动物适应性免疫系统通过修改单个B细胞的基因组来编码与特定抗原结合的抗体。在大多数哺乳动物中，抗体由重链和轻链组成，这两者通过V、D（针对重链）、J和C基因片段的顺序重排而产生。每条链包含三个互补决定区（CDR1-3），这些区域共同决定了抗原的特异性。某些重链和轻链更倾向于与特定的抗原结合。我们考虑了具有相同重链V基因和CDRH3氨基酸序列的B细胞对，这些细胞来自不同的捐赠者，也被称为公共克隆型。我们发现，对于未接触过抗原的原始抗体，它们使用相同轻链V基因的概率约为10%，而对于记忆（功能）抗体，这一比例约为80%。这种功能抗体的特性，我们称之为轻链一致性。我们还在捐赠者体内观察到相似的重链重复出现。因此，尽管原始抗体似乎是通过偶然性重复的，但功能抗体的重复出现揭示了V(D)J重排和免疫选择过程中令人惊讶的约束和确定性。对于大多数功能抗体，重链决定了轻链。