p-Akt and PI3K inhibitor – singe-cell feature values

Data in the paper "Cellular state landscape and herpes simplex virus type 1 infection progression are connected" by Pietilä et al., 2023:
- Single-cell mean intensities of p-Akt (Ser473), p-Akt (Thr308), 4E-BP, cleaved caspase 3, and ICP27 in HeLa and A549 cells and summaries of apoptotic HeLa cells
- A549_pAkt_Ser473_validation.csv (Supplementary Figure 12a)
- HeLa_pAkt_Ser473_validation.csv (Supplementary Figure 8a)
- HeLa_pAkt_Thr308_two_replicates.csv (Supplementary Figure 8b)
- HeLa_PI3KInhibitor_4EBP_secondReplicate.csv (Supplementary Figure 13b)
- HeLa_PI3KInhibitor_4EBP.csv (Figure 6c)
- HeLa_PI3KInhibitor_cleaved_caspase3_ICP27_secondReplicate.csv (Supplementary Figure 13d)
- HeLa_PI3KInhibitor_cleaved_caspase3_ICP27.csv (Figure 6e)
- HeLa_PI3KInhibitor_percentage_apoptotic_cells_secondReplicate.csv (Supplementary Figure 13c)
- HeLa_PI3KInhibitor_percentage_apoptotic_cells.csv (Figure 6d)

HeLa or A549 cells were mock- or HSV-1-infected (indicated by "condition") and either untreated or treated with a PI3K inhibitor (LY294002) or DMSO (indicated by "treatment"). After fixation, the markers were detected by immunofluorescence imaging. Cells from HSV-1-infected wells were classified into uninfected cells without infected neighbors, uninfected cells with infected neighbors, and infected cells (indicated by “classification_infection”) as described in the paper. Single-cell intensities are provided as background-subtracted mean intensity values for the whole-cell. Data cleanup, background subtraction of the intensity values, and classification of cells were applied as described in the paper. Experimental conditions are in duplicate or triplicate (indicated by “well_name”).