Modeling and therapeutic targeting of inflammation-induced hepatic insulin resistance using human iPSC-derived hepatocytes and macrophages

RNAseq was used to identify inflammation-related pathways activated in iPSC-derived hepatocytes by co-culture with iPSC-derived macrophages, thereby allowing for making inferences about cytokine mediators. Comparison of gene expression profile by RNAseq of iPSC-derived hepatocytes (n=3 per condition) after 24-hour co-culture with activated (M1) iPSC-derived macrophages or non-activated (M0) iPSC-derived macrophages or after mon-culture (C).