RNA-seq of mosue heart tissues overexpressed with Monkeypox virus (MPV) proteins H3L or A29L.

Here, we overexpressed Monkeypox virus (MPV) proteins H3L and A29L in mouse hearts, separately. We appliced bulk RNA-seq on heart muscle tissues (2 months after overexpression) and want to know gene expression patterns in control group and overexpression groups. Subsequent data analyses will help us to know whether and how H3L and A29L affect mosue heart. Overall design: Total RNAs were extracted and purified with miRNeasy Mini Kit (Qiagen). Three biological replicates were applied for the bulk RNA-seq.