Nox4 effect on mouse articular chondrocytes

Gene expression profiling of mouse articular chondrocytes infected with Ad-C or Ad-Nox4 In this study, we have attempted to explore the effects of Nox4 and have identified numerous genes which are involved in osteoarthritis pathogenesis. mouse articular chondrocytes were isolated from mouse femoral condyles and tibial plateaus cartilage tissue and infected with Ad-C(control) or Ad-Nox4 for 36 hours. Total RNA was extracted from primary mouse articular chondrocytes cultured with Ad-C (2 biological replicates) or Ad-Nox4 for 36 hours (2 biological replicates) and subjected to microarray analysis.