Effect of LINC01315 depletion and overexpression on proliferation of OMM2.3 uveal melanoma cells. Effect of LINC01315 depletion and overexpression on proliferation of OMM2.3 uveal melanoma cells

Cell proliferation disorders contribute to several diseases including cardiac developmental defects and tumours,and identifying pivotal factors that modulate the cell proliferation process is important. In this study, we identified a short peptide, YAPer-ORF, encoded by the long-stranded non-coding RNA gene LINC01315. we clarified the biological function of YAPer-ORF in promoting cell proliferation using gene manipulation and examined various cell lines including OMM2.3. In terms of the molecular mechanism, we found that YAPer-ORF regulates YAP intranuclear kinase PRP4K, while coercing GNAQ/11 mutants to undergo nuclear translocation and inhibit transcription of critical factors in the Hippo signalling pathway to synergistically activate YAP activity.We finally validated our findings in transgenic mice with cardiac-specific expression and nude mouse models of tumour formation. Together, the present study elucidates a novel regulatory mechanism of the Hippo signalling pathway and provides a new intervention target for disorders arising from dysregulation of the Hippo signalling pathway. Overall design: To investigate regulated action of YAPer-ORF on cell proliferation, we applied lentiviral infection to knockdown and overexpress target genes in uveal melanoma cell line OMM2.3.