Gene expression of cell populations sorted from exocrine cells infected with PNM (M3) or NGN3 and LacZ or REST. Gene expression of cell populations sorted from exocrine cells infected with PNM (M3) or NGN3 and LacZ or REST

Bulk MARS-seq analysis of gene expression identified genes induced by PNM or NGN3 that are inhibited by REST expression. Overall design: Primary exocrine cells were isolated from C57BL/6 mice and infected with Ad-M3-mCherry (PNM expressing) or Ad-NGN3-GFP and Ad-LacZ or Ad-REST viruses. Cell populations were FACS sorted according to mCherry and GFP expression. RNA from 4 biological replicates of these samples was used for library construction using Bulk MARS-seq protocol and sequenced.