Gut IgA Enhances Systemic IgG Responses to Pneumococcal Vaccines Through the Commensal Microbiota

We set up to characterize the global transcriptome of splenic follicular (FO) B cells from control wild type (Wt) and Igha-deficient (IgAKO) mice with the aim of gaining new insights into how translocated gut antigens may impair IgG production to vaccines in the absence of IgA. Splenic Marginal zone (MZ) B cells were also included in this study, as IgA deficiency impaired IgG responses to T-independent immunogens as well. RNA from sorted splenic MZ B cells and FO B cells were obtained from 4-5 age- and sex-matched wild type and IgA-deficient (IgAKO) mice.