A chromosome-level genome assembly of Hemiphyllodactylus yunnanensis

Approximately 60% of gecko species possess adhesive toepads equipped with specific adhesive setae on their digits, enabling them to climb vertical surfaces with ease. In this study, we present a high-quality chromosome-level genome assembly of gecko Hemiphyllodactylus yunnanensis generated by combining PacBio long-read sequencing and Hi-C data sequencing techniques. The resulting assembly comprises 1553.56 Mb across 841 scaffolds and 20 chromosomes, with a total length of 1420Mb. The contigs N50 and scaffold N50 values are 7.88 Mb and 86.91 Mb, respectively, accounting for 91.27% of the original genome length. Gene structure and functional annotation analysis revealed that the genome of H. yunnanensis contains 19,309 protein-coding genes, with an average gene length of 30,406.32 bp. The resulting genome assembly and annotation provide a genomic resource for future studies of H. yunnanensis and comparative genomics.