Gene expression and alternative splicing in human gastric cancer

27 sample pairs were conducted exon microarrays and 25 sample pairs were retain for our further alternative splicing analysis. 260 genes showed differential expression in more than 50% of our samples and 21 genes among them were validated by real-time PCR. Six genes with splicing events, which were firstly found in gastric cancer, were confirmed by RT-PCR. Keywords: Transcriptome analysis Total 49 gastric caners and corresponding normal mucosas were choose to reexamine the pathological information and procure pure cells using laser capture microdissection. 25 sample pairs passed microarray quality control and were retained for further alternative splicing analysis. One sample per array.