scRNAseq of mouse epidermis with and without 6h of IMQ treatment. scRNAseq of mouse epidermis with and without 6h of IMQ treatment

We report the application of single-cell-RNA sequencing technology for high-throughput profiling of primary mouse epidermal cells. We use a topical toll-like receptor 7 agonist, Imiquimod (IMQ) to activate the skin immune system. We find that the proportion of most of the cell types are conserved after IMQ treatment. We identify many interferon-sensitive genes that are induced after 6 hours of IMQ treatment. This study provides a deeper understanding of the cell type-specific anti-viral gene expression response to chemical modulators such as IMQ. Overall design: Examination of 2 treatment conditions (control shaved vs 6 hr of 1 % IMQ treatment applied topically onto shaved mouse dorsal back skin)