PBL Barley and AtPBS1 Alignment

Alignment of PBS1 and PBS1-like protein kinase amino acid sequences from barley and Arabidopsis, aligned by Clustal Omega. Barley sequences were obtained by a BLAST search using Arabidopsis PBLs as queries. This alignment was used to generate the phylogenetic tree in Figure 2A and S1 in Carter and Helm, et al 2018 (in submission).<br>From methods section:Sequence similarity searches were performed using BLASTp to gather barley amino acid sequences homologous to Arabidopsis PBS1 and PBS1-like proteins. First, AtPBL (1 to 27), BIK1, and other PBS1-homologous sequences were gathered by searching the Arabidopsis genome (TAIR10, GCA_000001735.1) with the AtPBS1 (OAO91748.1) amino acid sequence and by name search. Potential barley PBLs were collected by searching the barley protein database (assembly Hv_IBSC_PGSB_v2) with each Arabidopsis homologue and taking the top five hits derived from distinct genes. Amino acid sequences were aligned with Clustal Omega (Sievers et al., 2011). <br>