PRDM16 regulates the postnatal fate of embryonic radial glia via VCAM1-dependent mechanisms

Postnatally, a subpopulation of embryonic neural stem cells becomes adult neural stem cells, which primarily generate olfactory bulb interneurons in mice. Although embryonic and adult neural stem cells have been characterized, the mechanisms that regulate the transition from embryonic to adult neural stem cells remain poorly understood. We show that PR domain-containing 16 (Prdm16) promotes the transition from embryonic to adult neural stem cells. By single-cell RNA sequencing, it reveals postnatal persistence of radial glia in Prdm16 cKO mice. Overall design: The V-SVZ of the lateral walls of the lateral ventricles from one-month old (P31-33) mice were dissected according to published protocols (Mirzadeh et al., 2010). The V-SVZs from 2-3 mice including both males and females were combined as a biological replicate. 3, 3, and 1 biological replicates from control, Prdm16 cKO, and Prdm16-Vcam1 DKO were analyzed.