Hepatic lipopolysaccharide binding protein uncouples inflammation from fibrosis in MAFLD

MAFLD is a heterogenous spectrum disorder affecting 20% of the population. Inflammatory processes contribute to various stages of MAFLD and thought to instigate hepatic fibrosis. For this reason, targeting inflammation has been heavily nominated as an approach to mitigating liver fibrosis. Lipopolysaccharide binding protein is a secreted protein that plays an established role in innate immune responses. In a murine model of MAFLD, we used a liver-specific deletion of LBP model to study its role in hepatic inflammation. Single nucleus RNA-seq were applied to investigate the role of LBP in hepatic cell composition. The nuclei were isolated using frozen liver from WT and LBP-KO male mice that fed with FPC diet for 16 weeks. The snRNA-seq were applied to investigate the cell composition in the liver.