Target Capture Sequencing of SARS-CoV-2 Genomes Using the ONETest Coronaviruses Plus

We introduce a target capture next-generation sequencing methodology, the ONETest Coronaviruses Plus (Fusion Genomics Corp.), to sequence SARS-CoV-2 genomes and select loci of other respiratory viruses. We applied the ONETest on 70 respiratory samples (collected in Florida, USA between May and July, 2020), in which SARS-CoV-2 had been detected by a qualitative PCR assay. For 48 of the samples, we also applied the ARTIC protocol.