The effect of trichostatin A on porcine mesenchymal stem cells transcriptome

The use of histone acetyl-transferase inhibitors such as Trichostatin A (TSA) for epigenetic modulation of mesenchymal stem cells (MSCs) is an interesting approach in research involving somatic cell cloning of pigs and other mammalian species. Despite the effectiveness of TSA in cloning applications was already confirmed, the detailed mechanisms underlying this effect are not yet fully recognised, especially for pig MSCs. To add to this knowledge, in this study we performed a comprehensive transcriptome analysis using high-throughput RNA sequencing of pig bone-morrow derived MSCs, treated and untreated with TSA, and evaluated the effect of TSA administration on their transcription profile after 24 hours of in vitro culture. Subsequently, the stability of introduced epigenetic modifications was evaluated after another 50-72 h of culture without TSA.