Microarray expression profile of exosomal circRNAs from high glucose stimulated human renal tubular epithelial cells

we used microarray technology to construct a comparative circRNA profiling of exosomes isolated from cell supernatant of high glucose stimulated human proximal tubular epithelial cell line (HK-2 cells) and nomal controls, aimed to explore gene expression and the underlying molecular mechanism of exosomal circRNAs in DN development. High glucose induced gene expression in exosomes isolated from cell supernatant of human proximal tubular epithelial cell line (HK-2 cells) was measured at 30 hours after exposure to 30 mmol/L glucose. For the control group, HK2 cells were cultured in 5.5 mmol/L glucose for 30h. Three duplicates of each group were performed.