Aerobic exercise significantly alters Notch1 signaling in rats with Alzheimer's disease

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and a major cause of dementia. The aim of this study was to investigate the effect of aerobic training on the expression changes of Notch1, Rbpjk, Hes1 and Hey1genes in the hippocampus of Alzheimer rats. Methods: Forty, eight-week-old male Wistar rats were divided into four groups: control (n=10), exercise (n=10), Alzheimer's (n=10), Alzheimer’s + exercise (n=10). Endurance training was implemented with increasing intensity starting at 15 m/min in the first week and progressing to speeds of 16-20 m/min over the next five weeks with increased durations each week. After 6 weeks, animals were euthanized and hippocampus was collected, frozen and RNA was isolated to quantify Notch1, Rbpjk, Hes1 and Hey1 expression. All statistical analyses and graphs were conducted using SPSS and visualized using GraphPad Prism, with significance set at p