Next-generation RNA Sequencing and Transcriptome Comparison of Mouse Wild Type and Mesenchymal Tgfbr2 conditional knockout lungs of E15.5

Lung mesenchymal knockout of transforming growth factor, beta receptor II (Tgfbr2) will lead to lung malformation, including impaired lung branching and cystic lesion. In order to understand the underlying mechanisms, total RNA of wild type and mesenchymal Tgfbr2 knockout lungs were isolated and sequenced using the next-generation RNA sequencing technique. Tgfbr2 flox mice were crossed with Tbx4-rtTA/TetO-Cre transgenic mice and received doxycycline treatment from E6.5 to generate the lung mesenchyme-specific and inducible Tgfbr2 conditional knockout (CKO) mice. Lung mRNA profiles of E15.5 fetal wild type and Tgfbr2 conditional mice were then generated by deep sequencing, in triplicate.