Gut microbiome from mouse. mouse gut metagenome

Comparisons of the distal gut microbiota of genetically obese mice and their lean littermates, as well as those of obese and lean human volunteers have revealed that obesity is associated with changes in the relative abundance of the two dominant bacterial divisions, the Bacteroidetes and the Firmicutes. We have performed comparative metagenomic analyses to examine how that these changes affect the metabolic potential of the mouse gut microbiome. DNA was isolated from the distal gut (ceca) of eight-week old C57BL/6J obese (ob/ob) and lean (ob/+ and +/+) mice using a bead beater to mechanically disrupt cells, followed by phenol-chloroform extraction. 3730xl capillary- and GS20 pyro-sequencers were used to generate 199.8Mb of community DNA sequence. Finally, the cecal microbiota of C57BL/6J ob/ob and +/+ donors were transplanted into 8-9-week-old germ-free +/+ C57BL/6J recipients for 14 days. Cecal bacterial community structure was compared in donors and recipients by 16S-rRNA-gene-sequence-based enumeration.