Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster

#Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster

##Authors

Abhishek Meena, Alessio N. De Nardo, Komal Maggu, Sonja Sbilordo, Benjamin Eggs, Rawaa Al Toma Sho, Stefan Lüpold

## Citation of associated articleMeena, A., Maggu, K., De Nardo, A.N., Sbilordo, S.H., Eggs, B., Al Toma Sho, R., Lüpold, S., 2024. Life stage-specific effects of heat stress on spermatogenesis and oogenesis in Drosophila melanogaster. Journal of Thermal Biology 125, 104001. https://doi.org/10.1016/j.jtherbio.2024.104001

##OverviewThis dataset was collected to assess the impact of heat stress across various life stages on reproductive performance in Drosophila melanogaster. The study focuses on stage- and sex-specific reproductive metrics such as: mating success, fertility, fecundity, hatching success, which for females were summed over four days and for males were assayed for four consecutive 24-hour periods, each with a different female. 

The dataset is structured to analyze these outcomes at different temperatures and developmental stages, providing insights into sex- and stage-specific vulnerabilities to heat stress. 

The analyses are performed in an R Markdown (.Rmd) file, using data stored in a CSV file

##Contents

The dataset includes the following files:

Rscript.Rmd: An R Markdown file containing code for analyzing sex- and stage-specific reproductive fitness based on the data in Data.csv.Data.csv: A CSV file with the raw data used for the analysis.

##Data Description

Note: NA's in each data file represent missing data (data not available). 

The Data.csv file contains the following columns:

Unique.ID: Unique identifier for each experimental individual.

SampleID: Identifier for each treatment group.

Temperature: Thermal exposure temperature (measured in degrees Celsius) for 4 hours, with levels: 24.5°C, 28°C, 32°C, 36°C, and 38°C.

Lifestage: Developmental stage during which thermal treatment was applied.

Sex: Sex of the individual exposed to the thermal treatment.

Day: Days post adult treatment exposure.

Block: Experimental block identifier for randomized grouping.

Total: Total number of eggs laid within 24 hours of oviposition.

Hatched: Number of eggs that successfully hatched.

Unhatched: Number of eggs that did not hatch (derived from the difference between Total and Hatched).

##Usage

To analyze the dataset, open and run the respective R Markdown (Rmd) files in RStudio or any compatible R Markdown environment. The Rmd files contain all the necessary code to reproduce the analyses described in the overview.