Epithelial-mesenchymal transition markers screenedina cell-based model and validated in lung adenocarcinoma

The epithelial-to-mesenchymal transition (EMT) process in cancer enables the migratory and invasive capabilities associated with metastatic competence. The cancer microenvironment plays an important role in inducing the occurrence of EMT. A number of extracellular stimuli and associated signaling pathways have been identified to promote EMT, however, molecular features during the early stages of EMT are poorly understood. Here we have established a novel in vitro EMT-inducing system that mimi cs tumor microenvironment and allows for close monitoring of the EMT progress. Using RNA-seq and miRNA-seq, we analyzed the transcriptomic transitions accompanying the progression of EMT, and pinpointed several molecular features during early EMT of the lung carcinoma cells. CAF conditioned medium treated and control A549 cells were collected at six different time points (3h, 6h, 12h, 24h, 48h, 72h) for RNA-seq and miRNA-seq, two biological replicates for each sample.