ATAC-Seq of WT (KOS) and ICP4 null (n12) HSV-1 Productive Infection in MRC5 cells

MRC5 cells were mock-infected or infected with WT (KOS) or ICP4-null (n12) HSV-1 at an MOI of 10 PFU/cell. WT samples were harvested after two hours. Mock and n12 infected cells were harvested after four hours. ATAC-Seq was performed on 1E+6 cells which were isolated and tagmented using the Nextera DNA library prep kit. Illumina sequencing was carried out at Tufts University Core Facility using HiSeq 2500 to generate 50 bp single-end reads.