Plant tissue selection for nuclear DNA content estimation

A figure illustrating the differences in FCM histograms when alternative organ/tissue types of the same plant were used for DNA content estimation.
Conclusion:
Organ / tissue type selection can be used to lower the risk of incorrect DNA content estimates caused by high content of secondary metabolites interfering with fluorescent staining and/or tissue endopolyploidy. To maximize comparability, all tissue samples were collected from the same individual and analyzed on the same instrument. Lower quality of some FCM histograms was expected in the experiment (and reproduced in an unaltered form).