Hepatic ARID3A facilitates liver cancer malignancy by cooperating with CEP131 to regulate an embryonic stem cell-like gene signature

Huh 7 cells were infected with lentivirus or transfected with siRNA using Lipofectamine RNAiMAX to overexpress or knockdown indicated genes. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed using indicated antibodies to study the H3K9me2 status after certain treatment in Huh7 cells. Cleavage Under Targets and Tagmentation (CUT&Tag) was performed to explore the chromatin binding sites of ARID3A. Whole-transcriptome sequencing (RNA-Seq) was performed to identify the downstream target genes regulated by ARID3A or KDM3A in Huh 7 cells. Overall design: Huh 7 cells were infected with lentivirus or transfected with siRNA using Lipofectamine RNAiMAX to overexpress or knockdown indicated genes. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed using indicated antibodies to study the H3K9me2 status after certain treatment in Huh7 cells. Cleavage Under Targets and Tagmentation (CUT&Tag) was performed to explore the chromatin binding sites of ARID3A. Whole-transcriptome sequencing (RNA-Seq) was performed to identify the downstream target genes regulated by ARID3A or KDM3A in Huh 7 cells.