Expression of candidate genes by keratinocytes co-cultured with skin commensal

To investigate whether skin bacteria might influence the expression of selected genes, we co-cultured human keratinocytes with S. epidermidis, an abundant commensal in human skin and performed RNA sequencing analysis. Keratinocytes were cultured with and without the addition of Staphylococcus epidermidis cultures, six replicates in each group. Host RNA were isolated and RNA-Seq was performed to identify differential expression of selected genes.