RNA-seq for hand-microdissected glomeruli from Immunoglobulin A nephropathy patients

We collected glomeruli from biopsy specimens from IgA nephropathy patients with relatively preserved kidney function (eGFR ≥ 60 mL/min/1.73 m2 and urine protein-to-creatinine ratio < 3 g/g) and from normal kidney cortices by hand microdissection and performed RNA-seq. The transcriptomic profiling of IgA nephropathy glomerulus provide insights for intraglomerular pathophysiology of IgAN before reaching profound kidney dysfunction. RNA-seq with 14 IgA nephropathy patients and 10 normal kidney cortices isolated for renal cell carcinoma patients