Mechanistic Studies of the Regulation of Cardiomyocytes Spatial Distributions by Heterogeneous Erbb2 Expression

Purpose: To investigate the mechanism of how Notch-Erbb2 signaling coordinated the cardiomyocyte interactions to direct ventricular chamber morphogenesis. Methods: Approximately 1000 hearts dissected from 96 hpf Tg(myl7:mCherry) embryos were collected for RNA sequencing. Results: We found that the expression levels of most genes involved in PI3K-AKT signaling and actomyosin structure organization increased prominently. Conclusions: Notch prevents excessive trabeculation by intercepting Erbb2-PI3K signaling pathway. Overall design: 96 hpf Tg(myl7:mCherry) zebrafish embryos treated with DMSO or DAPT 100 µM were collected for RNA-seq.