Data for: Copper ions inhibit pentose phosphate pathway function in Staphylococcus aureus

To gain a better insight of how Cu ions toxify cells, metabolomic analyses were performed in S. aureus strains that lack the described Cu ion detoxification systems (ΔcopBL ΔcopAZ; cop-). Exposure of the cop- strain to Cu(II) resulted in an increase in the concentrations of metabolites utilized to synthesize phosphoribosyl diphosphate (PRPP). PRPP is created using the enzyme phosphoribosylpyrophosphate synthetase (Prs) which catalyzes the interconversion of ATP and ribose 5-phosphate to PRPP and AMP. Supplementing growth medium with metabolites requiring PRPP for synthesis improved growth in the presence of Cu(II). A suppressor screen revealed that a strain with a lesion in the gene coding adenine phosphoribosyltransferase (apt) was more resistant to Cu. Apt catalyzes the conversion of adenine with PRPP to AMP. The apt mutant had an increased pool of adenine suggesting that the PRPP pool was being redirected. Over-production of apt, or alternate enzymes that utilize PRPP, increased sens..., The glucose gluconate tab contains glucose v gluconate growth for Fig 3. Bacterial strains were grown at 37 °C in a microtiter plate containing a chemically defined medium with 11 mM glucose or 11 mM gluconate as a primary carbon source. 
The 13C ion counts tab contains the 13C counts utilized for Figure 2 and Figure s1. Metabolic analyses were conducted using biological triplicates. Overnight cultures were diluted into fresh TSB to OD600 0.1 and grown until OD600 of 0.5 in a flask. At this point, 5 mL of culture was transferred to individual 30 mL capacity culture tubes and exposed to 0 or 5 µM Cu(II) for three hours. Samples for the metabolite profiling were prepared as described previously (1). Briefly, 1 mL of the cells were pelleted, washed once with PBS, and resuspended in 1 mL of Methanol:Acetonitrile:Water (2:2:1) solution. Cells were lysed by bead beating (2 cycles, 40 s each, 6.0 m s-1) using a FastPrep homogenizer (MP Biomedicals) and 0.1-mm silica glass beads (MP Biomedicals...,