Comparison of digital PCR, agar proportion, real-time PCR, and PCR with Sanger sequencing.
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R = resistant. S = susceptible. Agar proportion resistance defined as >1% resistant colonies, and the actual percentage of resistant colonies is shown in parentheses. Digital PCR resistance defined as >1% of PCR reactions indicating mutant sequence, with the actual percentage of PCR reactions with mutant sequence shown in parentheses. Real-time PCR resistance defined as mixed populations of wild-type and mutant sequence. Sequencing defined resistance as evidence of mutant sequence by chromatogram.
创建时间：
2013-02-27
