Comparison of extracellular vesicle and filamentous cell transcriptomes of Ustilago maydis

The coding transcriptomes of filamentous cultures of the maize smut fungus Ustilago maydis and their extracellular vesicles (EVs) were compared. Protein-coding transcripts relatively enriched in EVs versus filament cells were identified and examined to identify potentially functional mRNA cargos of U. maydis EVs. Overall design: Poly(A)-enriched libraries were generated from four biological replicates each of Mock- (PBS) and RNase-treated extracellular vesicle preparations and the corresponding filamentous cells (4 biological replicates x 3 samples each). Sequencing was carried out on Illumina MiSeq platform with v3 chemistry (2x 150 bp paired-end sequencing).