Single-molecule real-time sequencing identifies massive full-length cDNAs and alternative-splicing events that facilitate comparative and functional genomics study in the hexaploid crop sweet potato

Equal amounts of different tissues (young leaves, mature leaves, shoots, stems, and roots ) from a diploid Ipomoea trifida plant were collected and pooled, and used for SMRT sequencing (the Pacific Bioscience RS II platform) and Illumina sequencing for error correction.