Exploring the diversity of Peziza echinospora (Ascomycota, Pezizomycota) and related species in Australia, with descriptions of the new species P. austroechinospora and P. meridionalis [ITS+28S+rpb2 alignment]

Supplementary material for: Vaughan LJ, Birch JL, Catcheside PS, Lemmond B, Truong C. 2026. Exploring the diversity of Peziza echinospora (Ascomycota, Pezizomycetes) and related species in Australia, with descriptions of the new species P. austroechinospora and P. meridionalis. Mycologia (in press). DOI:10.1080/00275514.2026.2616180.The following files are associated with the phylogenetic analyses in Vaughan et al. (2026):ITS+28S+rpb2 alignment as FASTA.ITS+28S+rpb2 maximum likelihood phylogeny treefile.ITS+28S+rpb2 Bayesian inference phylogeny NEWICK tree.ABSTRACTPeziza echinospora (Ascomycota, Pezizomycetes) is a species of cup-forming fungi that bears spiny, ellipsoid ascospores and is typically found on burnt ground as a saprotrophic species. It has a cosmopolitan distribution and has been reported from Australia based on morphological studies, but this has not been confirmed with molecular data. To address this knowledge gap and explore the phylogenetic diversity within the group, we investigated the species delimitation of P. echinospora and related species from southern Australia, using multilocus phylogenetic analyses of nuclear rDNA internal transcribed spacer ITS1-5.8S-ITS2 (ITS), partial 28S rRNA, and rpb2 (second largest subunit of RNA polymerase II) and morphological examination of fungarium specimens. Based on these analyses, we confirm the occurrence of P. echinospora in Australia and formally describe two new species, P. austroechinospora and P. meridionalis. Peziza austroechinospora is 20 typically found on burnt ground and has spiny ascospores that are larger and proportionately longer than those of P. echinospora. Peziza meridionalis has smooth ascospores, similarly to the closely related species P. subvesiculosa from the Northern Hemisphere, but with smaller ascospores and an ecology not associated with burnt soil. The occurrence of Peziza meridionalis as an endophyte on orchid roots is also discussed.