Gene expression profile of sorted microglia and CD206 border-associated macrophages obtained from mice

Brain macrophages have long been considered a homogenous population as determined by flow cytometry. We use scRNAseq to investigate the significant pheotypic heterogeneity of brain macrophages, especially under neurodegenerative disorders, based on their transcriptional profile. Microglia and CD206+ border-associated macrophages were isolated from 6-month-old healthy and APP(NL-G-F) mice, with FIT2-WT or FIT2-Mut, respectively, to study their transcriptome alteration during Alzheimer's disease progression.