Impact of level of nutritional dose of colostrum versus milk replacer in promoting 24 h gain, circulating lipid profile, and circulating levels of immunocrit, proteins, glucose, and free amino acids in neonatal gilt piglets

<p style="margin-bottom:11px"><span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif">The data provided here are lipidome, proteome, and amino acid data generated from neonatal piglets fed different levels of colostrum and milk replacer. We conducted multiple reaction monitoring (MRM) profiling of lipids in diet (colostrum and milk replacer) and serum from neonatal piglets, proteome analysis of diets (colostrum and milk replacer), and evaluation of the amino acid concentration of diet and serum using liquid chromatography mass spectrometry (LC-MS). These data are presented and discussed in a manuscript submitted to Journal of Nutrition. </span></span></span></span></span></span></p>

<p style="margin-bottom:11px"> </p>

<p style="margin-bottom:11px"><span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif">Neonatal piglets that do not receive an adequate amount of colostrum, the first milk produced from the mammary gland after parturition, have poor immunity and pre-weaning survivability (Theil et al., 2014). Piglets that survive past weaning, but had poor colostrum intake, have reduced growth and fertility compared to piglets that an adequate amount of colostrum (Bartol and Bagnell; 2012, Bartol et al., 2013; Mills et al., 2021, 2023). Our lab determined the adequate amount of colostrum a piglet needs to consume within the first 24 hours postnatal to be 20% of birth body weight (</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif">Suárez</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif">-Trujillo et al., 2020). Colostrum is distinct from mature milk produced later in the sow’s lactation and has different nutrient composition and levels of bioactive components compared to mature milk. Therefore, the objective of this study was to determine the impact of nutritional and bioactive level of colostrum or milk replacer feeding on piglet growth, circulating lipidome, amino acids, and immunocrit, the measure of immunoglobulins in serum. Neonatal female piglets were identified at birth, weighed, and were not allowed to suckle from the sow. Then, piglets were allocated to one of the following treatments: 1) pooled colostrum from a commercial sow farm fed at 20% of birth body weight (BBW) (COL20); 2) pooled colostrum fed at 10% of BBW (COL10); 3) Ralco milk replacer fed at 20% BBW (MR20); 4) milk replacer fed at 10% BBW (MR10); 5) piglets that stayed on the sow and suckled ad libitum colostrum (SOS); and 6) piglets received no food and immediately euthanized after birth (zero hour (ZH)). Piglets were bottlefed their dietary treatments every 2 hours for 24 hours, then euthanized. Blood was collected after euthanasia, and centrifuged to separate serum from whole blood. Serum was analyzed for lipidome and small metabolites using MRM profiling, free amino acids, immunocrit, glucose, and protein, but only the circulating lipidome, small metabolites, and free amino acids are presented as supplemental tables in the manuscript and uploaded to Purdue University Research Repository. The other parameters are shown in the manuscript. Samples of colostrum and milk replacer were collected throughout the study and analyzed for lipidome, proteome, energy, protein, fat, lactose, and free amino acids. </span></span></span></span></span></span></p>