Colon cleanout human gut V4 16S survey & shotgun metagenomic sequencing. human gut metagenome

Eight healthy human adults provided about 40 stool samples each (range 37-128 samples/subject) at daily or weekly intervals over about 22 weeks (range 20-55 weeks) surrounding a 1-day induced iso-osmotic diarrhea (colon cleanout) perturbation. The cleanout was identical to a common bowel preparation procedure prior to colonoscopy, involving the consumption of up to 4L of a polyethylene glycol-electrolyte solution until diarrhea was clear and watery. DNA was extracted from stool samples and investigated with V4 16S amplicon sequencing and with shotgun metagenomic sequencing (both sequencing modes using the Illumina HiSeq platform).