Trypanosoma brucei bloodstream form tagging: Targeted subcellular protein localisation.

Trypanosoma brucei bloodstream form tagging protein localisation data. Widefield epifluorescence microscope images of protein subcellular localisation in the bloodstream form life cycle stage of the unicellular eukaryotic pathogen Trypanosoma brucei by endogenous tagging with mNeonGreen (mNG). This master deposition includes a summary of the localisations, primer sequences and DOI indexing, provided in a directory structure analogous to the TrypTag genome-wide procyclic form project: https://doi.org/10.5281/zenodo.6862298 It does not include any microscopy data, which are spread over multiple Zenodo DOIs. Instead, this deposition and the raw and processed data directories include an index to each DOI. localisations.tsv Tab-delimited table, which can be opened in Excel, of localisation annotations for each gene tagged. Also includes primer sequences used, the 96 well plate in which tagging was carried out organised with one row per gene ID, with sets of columns for N and C terminal tagging. geneselection.tsv Tab-delimited table of criteria used for gene selection for tagging. This includes presence/absence of a Leishmania major or Trypanosoma cruzi ortholog, localisation and signal intensity by procyclic form tagging (TrypTag) and upregulation at mRNA level. id_doi_index.tsv Tab-delimited table listing all Trypanosoma brucei Lister 427 gene IDs, if tagging was attempted at the N or C terminus and, if so, the Zenodo DOI at which to find the microscopy data. To download data for a particular gene ID, find its entry in this table, go to the corresponding Zenodo DOI and download <plateid_date>.zip for the raw microscopy data or <plateid_date>_processed.zip for the processed microscopy data. In the latter, images are named by gene ID and tagged terminus. plate_doi_index.tsv Tab-delimited table listing all 96 plates which were part of the targeted bloodstream form tagging project and the Zenodo DOI at which the data can be found. Downloading the data from all of these Zenodo DOIs gives the full microscopy dataset. trypTag_BSF_master.zip Zip file containing the master directory structure for the targeted bloodstream form tagging project database. This contains all internal code which was used to build the bloodstream form tagging database from the raw microscopy data. readme.docx Documentation on data access and rebuilding the database using trypTag_BSF_master.zip