Nucleophile-disabled Lam16A mutant holds laminariheptaose (L7) in a cyclical conformation

Nucleophile-disabled Lam16A mutant holds laminariheptaose (L7) in a cyclical conformation Descriptor: PUTATIVE LAMINARINASE, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose Authors: Vasur, J, Kawai, R, Andersson, E, Widmalm, G, Jonsson, K.H, Hansson, H, Engstrom, A, Igarashi, K, Sandgren, M, Samejima, M, Stahlberg, J. Deposit date: 2009-06-24 Release date: 2010-01-26 Last modified: 2024-11-20 Method: X-RAY DIFFRACTION (1.4 Å) Cite: Synthesis of Cyclic Beta-Glucan Using Laminarinase 16A Glycosynthase Mutant from the Basidiomycete Phanerochaete Chrysosporium. J.Am.Chem.Soc., 132, 2010

失活亲核位点的Lam16A突变体将昆布七糖（laminariheptaose, L7）锁定为环状构象
描述符：推定昆布多糖酶（laminarinase），其结构为alpha-D-吡喃甘露糖-(1→2)-alpha-D-吡喃甘露糖-(1→6)-[alpha-D-吡喃甘露糖-(1→3)]alpha-D-吡喃甘露糖-(1→6)-beta-D-吡喃甘露糖-(1→4)-2-乙酰氨基-2-脱氧-beta-D-吡喃葡萄糖-(1→4)-2-乙酰氨基-2-脱氧-beta-D-吡喃葡萄糖，以及beta-D-吡喃葡萄糖-(1→3)-beta-D-吡喃葡萄糖-(1→3)-beta-D-吡喃葡萄糖-(1→3)-beta-D-吡喃葡萄糖-(1→3)-beta-D-吡喃葡萄糖-(1→3)-beta-D-吡喃葡萄糖-(1→3)-beta-D-吡喃葡萄糖
作者：Vasur J、Kawai R、Andersson E、Widmalm G、Jonsson K.H.、Hansson H、Engstrom A、Igarashi K、Sandgren M、Samejima M、Stahlberg J
提交日期：2009-06-24
发布日期：2010-01-26
最后修改日期：2024-11-20
检测方法：X射线衍射（分辨率1.4 Å）
引用文献：《利用来自担子菌黄孢原毛平革菌（Phanerochaete Chrysosporium）的昆布多糖酶16A糖基合成酶突变体合成环状β-葡聚糖》，《美国化学会志》，132卷，2010年