Identification of regulated genes associated with ketogenic diet treatment following SCI

To evaluate the changes in gene expression after spinal cord injury (SCI) and treatment with a Ketogenic Diet or a Control Diet (Refs., CD: F5960, Bioserv®; KD: F5848, Bioserv®), gene expression was studied 7 days after SCI surgery in rats. A total of 8 adult male Sprague-Dawley rats (~300 g at time of injury; Harlan Breeding Laboratory) were group-housed (21°C; 12 h: 12 h light:dark cycle). After a unilateral C5 laminectomy -dorsal processes of C4-C6 were held with a clamp and fixed in a frame tilted at a 22.5° angle and the cord was consutesd with a force of 150 Kdynes using the Infinite Horizon impactor- the animals were given ad libitum access to the 2 diets. Total RNA was isolated from 5-mm segment centered on the site of impact.为评估脊髓损伤（spinal cord injury, SCI）后以及生酮饮食（Ketogenic Diet, KD）或对照饮食（Control Diet, CD，参考文献中CD为F5960，购自Bioserv®；KD为F5848，购自Bioserv®）干预后的基因表达变化，本研究对脊髓损伤术后7天的大鼠开展基因表达分析。共计使用8只成年雄性斯普拉格-道利（Sprague-Dawley）大鼠（损伤时体重约300g，购自Harlan繁育实验室），采用群养方式饲养（环境温度21℃，光暗周期为12h:12h）。实验先实施单侧C5椎板切除术——将C4-C6的背侧突起以夹具固定于倾斜22.5°的支架中，使用Infinite Horizon脊髓撞击器以150千达因（Kdynes）的力造成脊髓挫伤——术后即刻让大鼠自由采食两种指定饮食。最终于损伤中心区域的5mm脊髓节段中提取总RNA。