Data of the six 28-sample relative standard curves used in the study.

NCq and E are presented with 95% confidence intervals. NCqs are not in absolute numbers but relative to N0 of the most concentrated sample in the dilution series. σ2: Random variation around regression lines. FCq: Fluorescence at threshold in arbitrary LightCycler units (×100). k is the proportionality factor between NCq and FCq (NCq/FCq) and Kpr BP the amplicon-length-corrected value. Parameters are comparable across days and targets since the relation between absolute and relative copy numbers is preserved.