Dataset for Structure−Activity Relationship of Lower Chlorinated Biphenyls and Their Human-Relevant Metabolites for Astrocyte Toxicity

The concentration-response relationship of three PCB mixtures, namely, A1016, A1254 and the cabinet mixture, and four lower-chlorinated biphenyls were assessed in C6 cells using the MTT cell viability assay. Along with the parent congeners, toxicity of their respective human-relevant metabolites was also similarly assessed in C6 cells. PCB52 and its human-relevant hydroxylated (4-OH-PCB52) and sulfated (4-OH-PCB52 sulfate) metabolites were found to be the most toxic compounds as confirmed via the LDH assay. Further, toxicity of PCB52, 4-OH-PCB52 and 4-OH-PCB52 sulfate was assessed in mouse primary glial cells and rat primary astrocytes and they were found to be similarly toxic in rat primary astrocytes. No sex-dependent differences in toxicity in rat primary astrocytes was observed when assessed by the MTT assay. Further, due to differences in their lipophilicity, partitioning of all the tested PCB congeners and their respective human-relevant metabolites was estimated using the equilibrium partitioning model.